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transparent GIF pixels

Hi all,
Is it possible to create transparent pixels? 

It appears from the help that you can, but only when you write png's to
disk. I have tried this and have been unsuccessful.

Anyways, i want to do it in a situation where i don't write and immediatly
read just to get my transparencies. I would like to make them transparent
while the image is in memory. And i would prefer gifs, not png's.

thanks for any clues or pointers.